WB result of Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb
Primary antibody: Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: T-47D whole cell lysate; LNCaP whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb (S-2694-53)
Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb (S-2694-53)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Androgen Receptor (AR-V7 Specific) |
| Synonyms | Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; DHTR; NR3C4; AR |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P10275 |
| Clone Number | S-2694-53 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | 22Rv1 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
Background
The Androgen Receptor splice variant 7 (AR-V7) is a constitutively active, ligand-binding domain–deleted form of the androgen receptor that retains the N-terminal transactivation domain and DNA-binding domain, enabling ligand-independent nuclear translocation and transcriptional activation of AR target genes; its expression, driven by alternative splicing or gene rearrangements, is markedly enriched in metastatic castration-resistant prostate cancer and functions as a key driver of resistance to second-generation anti-androgens such as enzalutamide and abiraterone by sustaining AR signaling even under systemic androgen suppression or pharmacologic AR inhibition.
Picture
Picture
Western Blot
