WB result of Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb
Primary antibody: Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: T-47D whole cell lysate; LNCaP whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb (S-2694-53)
Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb (S-2694-53)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Androgen Receptor (AR-V7 Specific) |
| Synonyms | Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; DHTR; NR3C4; AR |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P10275 |
| Clone Number | S-2694-53 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu |
| Positive Sample | 22Rv1 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| ChIP | 1:20-1:50 | Hu |
Background
The Androgen Receptor splice variant 7 (AR-V7) is a constitutively active, ligand-binding domain–deleted form of the androgen receptor that retains the N-terminal transactivation domain and DNA-binding domain, enabling ligand-independent nuclear translocation and transcriptional activation of AR target genes; its expression, driven by alternative splicing or gene rearrangements, is markedly enriched in metastatic castration-resistant prostate cancer and functions as a key driver of resistance to second-generation anti-androgens such as enzalutamide and abiraterone by sustaining AR signaling even under systemic androgen suppression or pharmacologic AR inhibition.
Picture
Picture
Western Blot
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 22-RV-1 treated with 10 nM DHT for 4h cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb (S-2694-53) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation. Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR. qPCR showed the enrichment of ABCB5, SPINK5 and SAT-α in Androgen Receptor (AR-V7 Specific)
Recombinant Rabbit mAb (S-2694-53)-immunoprecipitated sample.
