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Alexa Fluor® 488 Mouse Anti-Human CD90 Antibody (S-R538)

Alexa Fluor® 488 Mouse Anti-Human CD90 Antibody (S-R538)

Catalog Number: S0B5956 Application: FCM Reactivity: Human Conjugation: Alexa Fluor® 488 Brand: Starter
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$177.00 SGD
$177.00 SGD
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Product Details

Product Specification


Host Mouse
Antigen CD90
Synonyms Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; THY1
Location Cell membrane
Accession P04216
Clone Number S-R538
Antibody Type Mouse mAb
Isotype IgG1
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Hu

Background

Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

Picture

FC

Flow cytometric analysis of Human CD90 expression on Human PBMC (human peripheral blood mononuclear cells). Human peripheral blood mononuclear cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD34 antibody and either Alexa Fluor® 488 Mouse IgG1 Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD90 antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.