Flow cytometric analysis of CD90 expression on human PBMC (human peripheral blood mononuclear cells). Human peripheral blood mononuclear cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD34 antibody and either Alexa Fluor® 488 Mouse IgG1 Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD90 antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human/ Monkey CD90 Antibody (5E10)
Alexa Fluor® 488 Mouse Anti-Human/ Monkey CD90 Antibody (5E10)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD90 |
| Synonyms | Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; THY1 |
| Location | Cell membrane |
| Accession | P04216 |
| Clone Number | 5E10 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Isotype Control | S0B1519 |
| Application | FCM |
| Reactivity | Hu, RhMk, CyMk |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu, RhMk, CyMk |
Background
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
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Flow cytometric analysis of CD90 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with Alexa Fluor® 488 Mouse IgG1 Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti- Human CD90 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD90 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with Alexa Fluor® 488 Mouse IgG1 Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti- Human CD90 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
