Human IL-6 ELISA Kit(Advanced)
Human IL-6 ELISA Kit(Advanced)
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Product Specification
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-6 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-6 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin-6 (IL-6) is a multifunctional cytokine with α-helix structure, 22-28kDa phosphorylation and varying degrees of glycosylation. It plays an important role in the acute phase of disease response, inflammation, hematopoiesis, bone metabolism and cancer exacerbation. Mature human IL-6 has 183 amino acids and 41% homology to mouse and rat IL-6. Alternative splicing within IL-6 produces a variety of isomers, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8 + T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endotheliin), sympathetic neurons, cerebral cortical neurons, adrenal medullary chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult glial cells, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1B cells, and islet beta cells. IL-6 production is usually controlled by glucocorticoids, catecholamines, and secondary sex steroids, and is generally associated with cellular activation. IL-6 in normal human blood is in the range of 1 pg/mL, slightly increased during menstrual period, severely increased in the middle and late stages of some cancers, and significantly increased after major surgery. IL-6 elicits cellular signaling through a cell surface receptor, which is a heterodimeric complex consisting of a ligand-binding subunit (IL-6 receptor) and a signal-transferring subunit gp130. Binding of IL-6 to the IL-6 receptor triggers binding of the IL-6 receptor to gp130 and dimerization of gp130. gp130 is also a component of CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM receptors. Soluble IL-6 receptors are produced by alternative splicing and protease cleavage. Through trans-signaling mechanisms, soluble IL-6 and IL-6 receptor complexes can elicit responses in cells that lack IL-6 receptors on their surface but express gp130. The expression of IL-6 receptor is mainly restricted to hepatocytes, monocytes, lymphocytes and resting lymphocytes. Since the gp130 molecule is very widely expressed, trans-signaling enables a wider range of cell types to respond to IL-6. The soluble gp130 spliceosome prevents trans-signaling of IL-6/IL-6R, but not signaling by other cytokines using the gp130 molecule as a co-receptor. Together with tumor necrosis factor α (TNFα) and IL-1, the acute inflammatory response caused by IL-6 plays an almost unique role in fever and acute inflammatory response of the liver. It also plays an important role in the transformation of acute inflammation to acquired immunity or chronic inflammatory diseases. IL-6 dysregulation can promote chronic inflammation, such as obesity, insulin resistance, inflammatory bowel disease, inflammatory arthritis, and sepsis, often involving trans-signaling of IL-6. In the presence of transforming growth factor TGF-β, IL-6 plays an important role in the differentiation of naïve T cells into Th17 inflammatory cells. IL-6 regulates bone resorption and is a major contributor to inflammatory joint damage in rheumatoid arthritis by promoting the activity of Th17 inflammatory cells. IL-6 is involved in the formation and instability of atherosclerotic plaques. However, IL-6 also has anti-inflammatory effects, such as skeletal muscle secretion of IL-6 during physical exercise. As a growth factor of hematopoietic stem cells, it promotes and induces B cells to mature into plasma cells and immortality of multiple myeloma cells. IL-6 also promotes, but may not initiate, other inflammation-related carcinogenesis, such as colitis-related cancers. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 ℃. | |||||||||||||||||||||||||||
| Test Range | 1.65pg/mL-1200pg/mL |
