• Human FLT4 ELISA Kit
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Human FLT4 ELISA Kit

Human FLT4 ELISA Kit

Catalog Number: abs510040 Application: ELISA Reactivity: Human Conjugation:
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$368 USD
$368 USD
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Product Details

Product Specification

Theory This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate were sequentially added to microwells pre-coated with human vascular endothelial growth factor receptor 3 (VEGFR3; FLT4) capture antibody, incubated and washed in the middle, and colored by substrate TMB, which was converted to blue under the catalysis of peroxidase (HRP) and final yellow under the action of acid. There was a positive correlation between the depth of color and the human vascular endothelial growth factor receptor 3 (VEGFR3; FLT4) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated.
Source Human
Synonym Human Vascular Endothelial Growth Factor Receptor 4 (VEGFR3；FLT4) ELISA Kit
Detection Type Double antibody sandwich method
Description Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT4, is a protein encoded by the FLT4 gene. This gene encodes the tyrosine kinase receptors of vascular endothelial growth factors C and D, which are believed to be involved in lymphatic vessel formation and lymphatic endothelium maintenance. Mutations in this gene cause hereditary lymphedema type IA. It has been shown to interact with SHC1.
Composition
Name 9 6T match set Storage conditions after opening
Pre-coated 96-well plate 8 holes × 12 strips -20℃
Standard 2 sticks
-20 ℃, use on the day after reconstitution
Universal diluent
2 × 20 mL
2-8℃
Concentrated biotinylated detection antibody (100 ×)
120uL
-20℃
Concentrated enzyme conjugate (100 ×)
120uL
-20 ℃ (protected from light)
20 × washing solution
2 × 10 mL
2-8℃
Substrate (TMB)
10mL
2-8 ℃ (protected from light)
Stop liquid
6mL
2-8℃
Sealing film
4 sheets
without
Instructions
1 serving
without
General Notes 1. Carry out incubation in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Refrigerate reagents immediately after use.
2. Incorrect plate washing may lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the micropores to dry for too long throughout the process.
3. Clean the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value.
4. The substrate color development solution should be colorless, and the substrate solution that has turned blue cannot be used.
5. Avoid cross-contamination of reagents and samples to avoid wrong results.
6. Avoid direct exposure to strong light during storage and incubation.
7. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reagents in the kit.
8. Expired products cannot be used, and components with different item numbers and batch numbers cannot be mixed.
9. Recombinant proteins from sources other than the kit may not match the antibodies in this kit and are not recognized.
10. If the disease may be spread, all samples should be managed well, and the samples and testing devices should be handled according to the prescribed procedures.
Storage Temp. Store at 2-8 °C.
Test Range 0.31-20ng/mL