Dog HE4 ELISA Kit
Dog HE4 ELISA Kit
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$460 USD
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Product Details
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0-10uL, 5uL, 10-100uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: the whole sample collected in the serum separation tube at room temperature for 2 or at 4℃, centrifuge at 1000×g for 20, and take the, or store the at -20℃ or -80℃, but avoid repeated and thaw. Plasma: Collect the specimen using EDTA or heparin as a and centrifuge it 1000 × g for 15 at 2-8°C within 30 collection. The can be or stored at -20°C or -80°C, but avoid repeated and. Tissue : Rinse the tissue pre-chilled PBS (0.01, pH 7.4) to residual (red in the will affect results). and the. and the. the to the (generally 1:9 -to-ratio, 1 g sample 9 mL. The can be according and. It is to inhibitors to the) in a and on. To, the can be or and. the at 5000 × g for 5-10, and the can be. saliva other: at 1000xg 20, remove, and test. Pre-test: 1. the test from the 10 in and to. 2. the working: 1 mL to the, for 15 to, then to make ( 10). the: 10, 5, 1, 1, 1, 1, 1, and 0. : and 1. 1 and. and. for. the for. 3. of antibody working: the antibody 1000×g for 1 15 use. the 100 antibody a working with (10 + 1). and. 4. the working: 15 use, the 100 at 1000×g for 1. the 100 a working with ( 10 + 1). and. 5. the 1: 10 of 20 1 of ( from the may; it is; at for). and. 1. the from the after for 10. the in a and to. 2. : 1 or to the. 1 to the. with a at 37°C for. ( to the with the to the. will the on the. the the the the the. the for and the.) 3. Antibody: the and the. 1 of Antibody to. with a at 37°C for 60. 4. : the and 1 1 to. for 1, and on. this three (a can be). 5. Working: 1 Working to. with a at 37°C for 30. 6. : the and the as in 5. 7. : 1 (TMB) to, with a, at 37°C in the for 15. 8. : and 1 to. the at 450. results: 1. the the and and the the as a. the - the - on - graph, as the and the the. 2. the the the the, the and. the the the. 
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with epididymal protein 4 (HE4) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of epididymal protein 4 (HE4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Dog | |||||||||||||||||||||||||||||||||
| Synonym | Dog epididymal protein 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | HE4 is a protein encoded by the WFDC2 gene. It is a tumor marker for ovarian cancer, with a sensitivity of 80% at a cutoff of 150 pmol/L. It is a member of the WFDC domain family. The WFDC domain, also known as the WAP signature motif, contains eight cysteines that form four disulfide bonds in the protein core and function as a protease inhibitor in many family members. This gene is expressed in lung epithelial cells and has also been found in some ovarian cancers. The encoded protein is a small secreted protein. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, saliva and other biological fluids |
