• CHO HCP ELISA Kit
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CHO HCP ELISA Kit

CHO HCP ELISA Kit

Catalog Number: abs590005 Application: ELISA Brand: Absin
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$952 USD
$952 USD
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Product Details

Product Specification

Theory This method uses one-step enzyme-linked immunosorbent ELISA. Samples containing CHOK1 HCP can be simultaneously reacted with HRP-labeled goat anti-CHOK1 antibodies and anti-CHOK1 antibodies coated on enzyme plates to finally form a sandwich complex of solid phase antibodies-HCP-labeled antibodies. Unbound antigen antibodies can be removed by washing the plate. After the TMB substrate is added to the well to complete the reaction, the color development is stopped after the stop solution is added, and the OD or absorbance value of the reaction solution at 450/650 nm is read with a microplate reader. The OD value or absorbance value is proportional to the HCP content in the solution. From this, the HCP concentration in the solution can be calculated from the standard curve.
Description Host Cell Protein (HCP) secreted by CHO (Chinese Hamster Ovary) host cells is likely to cause contamination of biological products. Failure to adequately remove contaminants in the early stage of drug development may lead to a decrease in drug efficacy or induce an immune response. The kit can quantitatively detect the residue of host cell protein in bioengineering pharmaceuticals with high sensitivity, optimize the process flow and ensure the safety of biological products.
Product parameters:
1. Sensitivity: 1ng/mL
2. Detection range: 3-100ng/mL
3. Precision: intra-batch coefficient of variation ≤ 10%, inter-batch coefficient of variation ≤ 15%
4. HCP coverage: more than 80%
5. Specificity: This kit is universal because it can specifically react with CHOK1 HCP without relying on the purification process
Composition
Serial number form Concentration Specifications Storage conditions
1 CHOK1 HCP Standard 0.5 mg/mL 70uL/tube ≤-20℃
2 Anti-CHO HCP-HRP 0.5 mg/mL 60uL/tube ≤-20 ℃, protected from light
3 TMB NA 12mL/vial 2-8 ℃, protected from light
4 20 × PBST 0.05% NA 15mL/vial 2-8℃
5 Stop liquid NA 12mL/vial Room temperature
6 Enzyme labeled plate sealing membrane NA 3 sheets Room temperature
7 BSA NA 1g 2-8℃
8 High adsorption 96-well prediction enzyme plate NA 1 piece 2-8℃
General Notes The termination solution is 2M sulfuric acid, please handle it carefully to prevent splashes!
Instructions This kit is used to quantitatively detect the residual content of CHOK1 host cell protein in a sample.
Storage Temp.

Components 1 and 2 are stored at ≤-20 °C; Components 5 and 6 are stored at room temperature; Components 3, 4, 7, 8 are stored at 2-8 °C. Valid for 12 months.