Trypsin, Mass Spectrometry Grade

Catalog Number: UA070085 Brand: UA BIOSCIENCE

Price:

$85 USD

Size: 100µg 200µg

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Synonyms	Trypsin(pig); porcine pancreas; Proenzyme; Parenzymol; PseudotrypsinTryptar; Trypure; Trypsin，Mass Spectrometry Grade
Expression System	Pichia pastoris
Molecular Weight	24±2 kDa (Reducing)
Purity	＞98% by SDS-PAGE
Tag	No Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	2mM HCl，2mM Ca2+，ph3.0 @ 25°C
Reconstitution	Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of 2mM HCl.
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	Olsen, J. V . Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues[J]. Molecular & Cellular Proteomics, 2004, 3(6):608-614. Chi W J. Medium Optimization and Application of Affinity Column Chromatography for Trypsin Production from Recombinant Streptomyces griseus[J].Journal of Microbiology & Biotechnology, 2009, 19(10):1191-1196.

Background

Trypsin is a serine protease commonly found in the vertebrate digestive system that hydrolyzes proteins and is secreted in the pancreas as inactive trypsinogen. Its site of cleavage of the peptide chain is located primarily at the carboxyl terminus of lysine or arginine (except when both are immediately followed by proline). Trypsin consists of two subunits, the alpha subunit (with 2 polypeptide chains forming it) and the beta subunit (with 1 polypeptide chain forming it). Its hydrolytic activity can be inhibited by a variety of inhibitors, including 1) organophosphorus compounds, such as isopropyl fluorophosphate; 2) natural trypsin inhibitors derived from pancreas, soybeans, green beans, and egg whites; 3) silver ions; and 4) specific protease inhibitors, such as AEBSF, anti-protease, peptidylase, DFP, leucoinhibitory peptidase, PMSF, TLCK, etc.

Components

Trypsin，Mass Spectrometry Grade lyophilized
2*Reaction buffer:，100 mM Tris-HCl 、40 mM CaCl2 pH 8 @ 25°C

Protocol

1 Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of 2mM HCl
2 Set-up a typical reaction as follows
1）Add the following components in sequence

Components	Volume
Substrate protein	5 μl (about 2 ug)
ddH2O	4.5μl
2*Reaction buffer	10μl
100 ng/μl Tyrpsin	0.5 μL

2）Incubate at 37°C for 2~4h

Guidelines

1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.

2. Storage buffer:2mM HCl, pH3.0.

Unit Definition

In a 3.2ml reaction system using BAEE as substrate, the UV absorption at 253nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit