WB result of ROCK1 Recombinant Rabbit mAb
Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
ROCK1 Recombinant Rabbit mAb (S-1304-153)
ROCK1 Recombinant Rabbit mAb (S-1304-153)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ROCK1 |
| Synonyms | Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; ROCK-I; p160 ROCK-1 (p160ROCK) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q13464 |
| Clone Number | S-1304-153 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Jurkat, NIH/3T3, C6 |
| Predicted Reactivity | Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| ICC | 1:500 | Hu, Ms |
Background
ROCK1 (Rho-associated coiled-coil kinase 1) is a serine/threonine protein kinase that plays a crucial role in various cellular processes, including cell proliferation, migration, adhesion, and cytoskeletal remodeling. ROCK1 is involved in the regulation of the actin cytoskeleton and stress fiber formation. It achieves this by phosphorylating and modulating the activity of a range of actin-binding proteins and intermediate filament proteins. The kinase activity of ROCK1 is regulated by its interaction with the small GTPase RhoA. When RhoA is activated, it binds to the Rho-binding domain (RBD) of ROCK1, leading to a conformational change that relieves the auto-inhibition of the kinase domain and enhances ROCK1's catalytic activity. ROCK1 has been implicated in the pathogenesis of several diseases, including cancer, cardiovascular diseases, and neurological disorders. In cancer, ROCK1 has been shown to promote cell proliferation and survival, making it a potential therapeutic target for cancer treatment. In the context of cardiovascular diseases, ROCK1 activity has been associated with hypertension and pulmonary hypertension, as well as the regulation of vascular tone and remodeling.
Picture
Picture
Western Blot
WB result of ROCK1 Recombinant Rabbit mAb
Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
WB result of ROCK1 Recombinant Rabbit mAb
Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
IP
ROCK1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ROCK1 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using ROCK1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: ROCK1 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 158 kDa
Observed MW: 160 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- ROCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- ROCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
