Mouse mAb IgM, κ Isotype Control (S-844-28)

WB result of Mouse mAb IgM, κ Isotype Control 
Primary antibody: Mouse mAb IgM, κ Isotype Control at 1/100 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution

Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

IHC shows negative staining in paraffin-embedded human tonsil. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows negative staining in paraffin-embedded rat spleen. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows negative staining in HeLa cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

ICC shows negative staining in C2C12 cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

ICC shows negative staining in C6 cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).