Flow cytometric analysis of BALB/c mouse splenocytes labelling Mouse H-2Kd antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse IgG2a, κ (Black) Isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Mouse Anti-Mouse H-2Kd Antibody (S-R647)
Mouse Anti-Mouse H-2Kd Antibody (S-R647)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Mouse H-2Kd |
| Synonyms | H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K1; H2-K |
| Location | Membrane |
| Accession | P01902 |
| Clone Number | S-R647 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.
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