Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling L-selection antibody at 1/1000 dilution (0.01 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: HeLa
L-selection Recombinant Rabbit mAb (S-1164-30)
L-selection Recombinant Rabbit mAb (S-1164-30)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | L-selection |
| Synonyms | CD62 antigen-like family member L; Leukocyte adhesion molecule 1 (LAM-1); Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1 (LECAM1); Lymph node homing receptor; TQ1; gp90-MEL; CD62L; SELL; LNHR, LYAM1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P14151 |
| Clone Number | S-1164-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM, ICC |
| Reactivity | Hu |
| Positive Sample | Jurkat |
| Predicted Reactivity | Pt |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:100 | Hu |
Background
L-selectin, also known as CD62L, is a type-I transmembrane glycoprotein and cell adhesion molecule that is expressed on most circulating leukocytes. Its function in leukocyte trafficking is highlighted by its role in lymphocyte homing to secondary lymphoid tissues. It mediates the tethering and rolling of lymphocytes on high endothelial venules (HEVs) in peripheral lymph nodes via interactions with glycoproteins expressing sLex epitopes. This process is essential for the proper functioning of the immune system, allowing for the recirculation of lymphocytes and their migration to sites of inflammation. In addition to its role in immune cell trafficking, L-selectin has been implicated in the adhesion of blastocysts to the endometrium during the implantation process in the female reproductive tract. It also plays a role in the recruitment of leukocytes during inflammation and in the homing of lymphocytes.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Jurkat cells (top panel) and negative staining in HeLa cells (below panel). Anti-L-selection antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
