Flow cytometric analysis of mouse thymocytes labeled with Rat IgG1 Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo rat IgG1 Isotype control, anti-trinitrophenol
Invivo rat IgG1 Isotype control, anti-trinitrophenol
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-3599 |
| Antibody Type | Rat mAb |
| Application | In vivo control |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
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FC
Flow cytometric analysis of mouse thymocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Rat IgG1 Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
