ICC shows positive staining in Histone H3-Glu-Glu Tag transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- Glu-Glu tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Glu-Glu tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-905-4)
Glu-Glu tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-905-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glu-Glu tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-905-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:50 | Species Independent |
Background
The Glu-Glu tag is a labeling or marker sequence specific to the Glu-Glu dipeptide, consisting of two consecutive glutamic acid residues. This tag is commonly utilized in biological research and experiments for the purposes of tagging and identifying proteins or antibodies, allowing for their tracking and analysis within complex biological samples.
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Immunocytochemistry
