Flow cytometric analysis of Mouse CD105 expression on bEnd.3 cells. Cells from the bEnd.3 (Mouse brain endothelioma, Right) or NIH/3T3 (Mouse embryonic fibroblast, Left) was stained with FITC Rat IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Rat Anti-Mouse CD105 Antibody (Red line histogram) at 1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse CD105 Antibody (S-R529)
FITC Rat Anti-Mouse CD105 Antibody (S-R529)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD105 |
| Synonyms | Endoglin; Cell surface MJ7/18 antigen; Eng; Edg |
| Location | Cell membrane |
| Accession | Q63961 |
| Clone Number | S-R529 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | bEnd.3 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
Endoglin (ENG) is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex. It is also commonly referred to as CD105, END, FLJ41744, HHT1, ORW and ORW1. Endoglin has been found to be an auxiliary receptor for the TGF-beta receptor complex. It thus is involved in modulating a response to the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, BMP-7 and BMP-9. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. In humans endoglin may be involved in the autosomal dominant disorder known as hereditary hemorrhagic telangiectasia (HHT) type 1.
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