2μg (R: reducing condition, N: non-reducing condition).
CEACAM-7 His Tag Protein, Human
CEACAM-7 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CEA, CEACAM 7, CGM 2, CGM2 |
| Accession | Q14002 |
| Amino Acid Sequence | Thr36-Ser242, with C-terminal 8*His TNIDVVPFNVAEGKEVLLVVHNESQNLYGYNWYKGERVHANYRIIGYVKNISQENAPGPAHNGRETIYPNGTLLIQNVTHNDAGIYTLHVIKENLVNEEVTRQFYVFSEPPKPSITSNNFNPVENKDIVVLTCQPETQNTTYLWWVNNQSLLVSPRLLLSTDNRTLVLLSATKNDIGPYECEIQNPVGASRSDPVTLNVRYESVQASGGGSHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 33-55kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Tchoupa, A.K. et al. (2014) Cell Commun. Signal. 12:27. |
Background
Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM-7), also known as CGM2, is an approximately 40 kDa GPI-anchored glycoprotein in the CEACAM family of adhesion molecules. Mature human CEACAM-7 consists of two Ig-like domains followed by the GPI anchor. Alternative splicing generates a short isoform that lacks the second Ig-like domain. CEACAM-7 is preferentially expressed on the luminal surface of epithelial cells near the mouth of colonic crypts and on pancreatic ductal epithelial cells. It is down-regulated during colorectal adeno
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SDS-PAGE
ELISA
Immobilized CEACAM-7 His Tag Protein, Human (Cat. No. UA010637) at 2.0μg/mL (100μL/well) can bind CEACAM-3/CD66d Fc Chimera Protein, Human (Cat. No. UA010277) with EC50 of 17.41-29.47ng/mL.
