CD45R⁺ cells were isolated from the single-cell suspension using CD45R Nanobeads, an S or L Separation Column, and a magnetic separator. Subsequently, fluorescent staining was performed with Alexa Fluor® 488 Rat Anti-Mouse CD45R/B220 Antibody (RA3-6B2) (STD Cat: S0B5952), and the live cell population was gated for analysis.
CD45R Nanobeads, mouse（RUO）
CD45R Nanobeads, mouse（RUO）
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Product Details
Product Specification
| Format | Dextran-coated iron tetroxide nanomagnetic beads conjugated with an anti-mouse CD45R monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs 1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs |
| Principle of Separation | MethodCD45R Nanobeads, mouseMagnetic labeling of CD45R-positive cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45R cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD45R cells. After removing the separation column from the magnetic field, the magnetically captured CD45R-positive cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD45R cells must be further separated using a second column. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD45R Nanobeads, mouseDesigned for positive selection or depletion of B cells from lymphoid tissues, peripheral blood, or bone marrow. The CD45R antigen is expressed throughout the entire development of B lymphocytes, starting from the early pro-B cell stage, and is downregulated upon terminal differentiation into plasma cells. CD45R is commonly used as a pan-B cell marker. It is also expressed on a small subset of dendritic cells (plasmacytoid dendritic cells).
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Validation Data
