Flow cytometric analysis of mouse primary splenocytes labelling CD45 antibody at 1/2000 dilution (0.1 μg)/ (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Gated on total viable cells.
CD45 Recombinant Rat mAb (S-R477)
CD45 Recombinant Rat mAb (S-R477)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD45 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC, Lymphocyte antigen 5 (Ly-5) |
| Location | Cell membrane |
| Accession | P06800 |
| Clone Number | S-R477 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:2000 | null |
Background
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
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