WB result of BMAL1 Recombinant Rabbit mAb
Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 70 kDa
BMAL1 Recombinant Rabbit mAb (S-1918-155)
BMAL1 Recombinant Rabbit mAb (S-1918-155)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BMAL1 |
| Synonyms | Basic helix-loop-helix ARNT-like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5 (bHLHe5); Member of PAS protein 3; ARNTL; BHLHE5; MOP3; PASD3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O00327 |
| Clone Number | S-1918-155 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SH-SY5Y, PC-3, Raji, NIH/3T3, mouse liver, rat liver |
| Predicted Reactivity | Or,Hr,Hm,Ck |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
BMAL1 (Brain and muscle ARNT-like 1, also known as ARNTL) is a bHLH/PAS-domain transcription factor that forms a heterodimer with CLOCK (or NPAS2) to drive the transcription of clock-controlled genes (e.g., Per and Cry) via E-box elements, thereby operating as the positive limb of the circadian transcription-translation feedback loop essential for generating ~24-hour molecular rhythms; it is the only clock gene whose loss abolishes circadian rhythmicity in mammals and whose disruption is linked to a spectrum of disorders including metabolic syndrome, psychiatric and neurodegenerative diseases, infertility, and altered sleep, while also regulating adipogenesis, immune responses, embryonic development, and protein synthesis through interactions with metabolic and signaling pathways.
Picture
Picture
Western Blot
WB result of BMAL1 Recombinant Rabbit mAb
Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 70 kDa
WB result of BMAL1 Recombinant Rabbit mAb
Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 70 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- BMAL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- BMAL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
