Flow cytometric analysis of Mouse CD45 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with either Alexa Fluor® 488 Rat IgG2b Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Rat Anti-Mouse CD45 Antibody (Red line histogram) at 1.25 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse CD45 Antibody (S-R477)
Alexa Fluor® 488 Rat Anti-Mouse CD45 Antibody (S-R477)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD45 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC |
| Location | Cell membrane |
| Accession | P06800 |
| Clone Number | S-R477 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume |
Background
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
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