WB result of USP1 Recombinant Rabbit mAb
Primary antibody: USP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100 kDa
USP1 Recombinant Rabbit mAb (S-2813-10)
USP1 Recombinant Rabbit mAb (S-2813-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | USP1 |
| Synonyms | Ubiquitin carboxyl-terminal hydrolase 1; Deubiquitinating enzyme 1 (hUBP); Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O94782 |
| Clone Number | S-2813-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HepG2, HEK-293, A20, mouse testis, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
USP1 (ubiquitin-specific protease 1) is a cysteine protease deubiquitinase that forms a tight complex with its activator UAF1 to remove monoubiquitin from key DNA-repair substrates—FANCD2/FANCI in the Fanconi-anemia pathway and PCNA during translesion-synthesis—thereby licensing accurate interstrand-crosslink repair, replication-fork stability, and genome integrity, while its frequent overexpression in cancers drives chemoresistance by also stabilizing oncogenic proteins such as ID1-3, EZH2, TAZ and survivin, making USP1 a promising therapeutic target.
Picture
Picture
Western Blot
WB result of USP1 Recombinant Rabbit mAb
Primary antibody: USP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100 kDa
WB result of USP1 Recombinant Rabbit mAb
Primary antibody: USP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100 kDa
