WB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
Transgelin 2 Recombinant Rabbit mAb (S-2809-73)
Transgelin 2 Recombinant Rabbit mAb (S-2809-73)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Transgelin 2 |
| Synonyms | Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homolog; KIAA0120; TAGLN2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoskeleton |
| Accession | P37802 |
| Clone Number | S-2809-73 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, HeLa, K562, HEK-293, NIH/3T3, mouse spleen, rat spleen, COS-7 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:5000 | Hu |
Background
Transgelin 2, also known as TAGLN2 or SM22β, is a 22-kDa actin-binding protein belonging to the calponin family, widely expressed in both smooth muscle and non-muscle cells, where it stabilizes and remodels the actin cytoskeleton by cross-linking and bundling F-actin, thereby regulating cell shape, motility, immune synapse formation, and vesicle trafficking; notably, it is markedly up-regulated during dendritic-cell maturation and T-cell activation, promotes tumor growth, invasion, and drug resistance in many cancers, yet exogenous cell-permeable transgelin-2 peptides can enhance dendritic-cell homing to lymph nodes and potentiate anti-tumor immunity, highlighting its context-dependent roles as both an oncogenic factor and a potential immunotherapeutic agent.
Picture
Picture
Western Blot
WB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
WB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
WB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
