WB result of TPH-1 Recombinant Rabbit mAb
Primary antibody: TPH-1 Rabbit mAb at 1/2000 dilution
Lane 1: 293T transfected with empty vector whole cell Lysate 10 µg
Lane 2: 293T transfected with human TPH-1 whole cell Lysate 10 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 48 kDa
TPH-1 Recombinant Rabbit mAb (S-2287-143)
TPH-1 Recombinant Rabbit mAb (S-2287-143)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TPH-1 |
| Synonyms | Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH; TPRH; TRPH; TPH1 |
| Immunogen | Synthetic Peptide |
| Accession | P17752 |
| Clone Number | S-2287-143 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Predicted Reactivity | Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
Background
TPH-1 (tryptophan hydroxylase-1) is a 51-kDa, biopterin-dependent and rate-limiting enzyme that catalyzes the hydroxylation of L-tryptophan to 5-hydroxytryptophan, thereby initiating peripheral serotonin synthesis in enterochromaffin cells, platelets, and mast cells; it is a homodimer whose activity is regulated by phosphorylation, allosteric cAMP-dependent mechanisms, and interaction with 14-3-3 proteins, and genetic or post-translational dysregulation of TPH-1 has been implicated in gastrointestinal disorders, cardiovascular fibrosis, and mood-related diseases.
Picture
Picture
Western Blot
