WB result of Tim23 Recombinant Rabbit mAb
Primary antibody: Tim23 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: SK-BR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
This blot was developed with high sensitivity substrate
Tim23 Recombinant Rabbit mAb (S-2241-105)
Tim23 Recombinant Rabbit mAb (S-2241-105)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Tim23 |
| Synonyms | Mitochondrial import inner membrane translocase subunit Tim23; TIM23; TIMM23 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | O14925 |
| Clone Number | S-2241-105 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, SK-OV-3, Jurkat, MCF7, SK-BR-3, NIH/3T3, mouse heart, PC-12, rat heart, COS-7 |
| Predicted Reactivity | Bv, Or, Xe |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Tim23 is an essential subunit of the TIM23 translocase in the mitochondrial inner membrane, where it serves as both a structural scaffold and a voltage-gated component that recognizes presequences of incoming precursor proteins in the intermembrane space, triggers channel opening in response to membrane potential, and—together with Tim17, Tim44, mtHsp70 and other motor proteins—forms a highly conserved multiprotein machine that imports ~1,000 matrix-destined and inner-membrane proteins from the cytosol into mitochondria, thereby undergirding organellar biogenesis and cellular energy metabolism.
Picture
Picture
Western Blot
WB result of Tim23 Recombinant Rabbit mAb
Primary antibody: Tim23 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
This blot was developed with high sensitivity substrate
WB result of Tim23 Recombinant Rabbit mAb
Primary antibody: Tim23 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
This blot was developed with high sensitivity substrate
WB result of Tim23 Recombinant Rabbit mAb
Primary antibody: Tim23 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
This blot was developed with high sensitivity substrate
