WB result of SREBP2 Recombinant Rabbit mAb
Primary antibody: SREBP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 60 kDa
SREBP2 Recombinant Rabbit mAb (S-2701-1)
SREBP2 Recombinant Rabbit mAb (S-2701-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SREBP2 |
| Synonyms | Sterol regulatory element-binding protein 2; SREBP-2; Class D basic helix-loop-helix protein 2 (bHLHd2); Sterol regulatory element-binding transcription factor 2; BHLHD2; SREBF2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane |
| Accession | Q12772 |
| Clone Number | S-2701-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Mk |
| Positive Sample | HeLa, HepG2, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
| IF | 1:500 | Hu |
Background
Sterol regulatory element-binding protein 2 (SREBP2) is a membrane-bound transcription factor that acts as the master sensor and amplifier of cellular cholesterol demand: in cholesterol-replete ER membranes it is held inactive through association with SCAP and Insig, but when cholesterol falls SCAP escorts SREBP2 to the Golgi where site-1 and site-2 proteases release its ~480-amino-acid N-terminal fragment; this mature form enters the nucleus, binds sterol response elements, and drives expression of nearly every gene required for cholesterol acquisition (HMGCR, HMGCS, LDLR) and efflux control (ABCA1, ABCG5/8), while also cross-talking with SREBP1c to coordinate overall lipid homeostasis, making SREBP2 the central switch that keeps mammalian cell membranes optimally functional.
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Picture
Western Blot
WB result of SREBP2 Recombinant Rabbit mAb
Primary antibody: SREBP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 58 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-SREBP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-SREBP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SREBP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SREBP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-SREBP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded human ovarian cancer. Anti- SREBP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human breast cancer. Anti- SREBP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
