WB result of SLC10A1/NTCP1 Recombinant Rabbit mAb
Primary antibody: SLC10A1/NTCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Low expression control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 40 kDa
SLC10A1/NTCP1 Recombinant Rabbit mAb (S-2844-23)
SLC10A1/NTCP1 Recombinant Rabbit mAb (S-2844-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC10A1/NTCP1 |
| Synonyms | Hepatic sodium/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide (NTCP6 Publications); Solute carrier family 10 member 1 (SLC10A1); NTCP |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q14973 |
| Clone Number | S-2844-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, Caco-2, mouse kidney, mouse liver, rat kidney, rat liver |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
Background
The SLC10A1 gene encodes the sodium/bile acid cotransporter NTCP1, a 349-amino-acid, 7-transmembrane-domain glycoprotein that is almost exclusively expressed on the basolateral membrane of hepatocytes, where it uses the inward Na⁺ gradient to mediate high-affinity uptake of conjugated bile acids and, notably, also serves as the principal entry receptor for hepatitis B and D viruses, while additional substrates including steroid sulfates, thyroid hormones, and certain drugs link NTCP1 to systemic bile acid homeostasis, lipid metabolism, and pharmacokinetics, and inherited or acquired dysregulation of the transporter produces cholestatic disorders that are increasingly targeted by small-molecule or siRNA therapeutics.
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Western Blot
WB result of SLC10A1/NTCP1 Recombinant Rabbit mAb
Primary antibody: SLC10A1/NTCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Negative control: mouse brain lysate
Low expression control: mouse kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 40 kDa
WB result of SLC10A1/NTCP1 Recombinant Rabbit mAb
Primary antibody: SLC10A1/NTCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Negative control: rat brain lysate
Low expression control: rat liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 40 kDa
WB result of SLC10A1/NTCP1 Recombinant Rabbit mAb
Primary antibody: SLC10A1/NTCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 40 kDa
