S-RMab® MUC1 Recombinant Rabbit mAb (SDT-776-49)

Catalog Number: S0B2284 Application: WB,IHC-P,ICC,IP,IF,FCM Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 500μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	MUC1
Synonyms	Mucin-1, MUC-1, CD227, CA 15-3, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, KL-6, PEMT, PUM, PEM, EMA, Tumor-associated mucin
Immunogen	Synthetic Peptide
Location	Cytoplasm, Secreted, Cell membrane
Accession	P15941
Clone Number	SDT-776-49
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC, IP, IF, ICFCM
Reactivity	Hu
Predicted Reactivity	Pr
Purification	Protein A
Concentration	0.25 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500
IHC-P	1:1000
IP	1:25
ICFCM	1:250
ICC	1:125
IF	1:125

Background

MUC1 is a transmembrane protein normally expressed in glandular or luminal epithelial cells and hematopoietic cells and absent in skin epithelium and mesenchymal cells. Apical surface of almost all glandular and ductal epithelial cells, including respiratory, gastrointestinal, urogenital, hepatobiliary tracts, sebaceous and salivary glands. MUC1 is expressed in the central cells of pancreatic acini, intercalary duct epithelium, and interlobular duct epithelium, but not in the acinar epithelium, main pancreatic duct epithelium, and pancreatic islet epithelium. Overexpression is common in malignancies and leads to tumorigenesis. Increased expression is associated with poor prognosis of most carcinomas, including those involving breast, cervical, pancreatic, colorectal.

Picture

Western Blot

WB result of S-RMab® MUC1 Rabbit mAb
Primary antibody: S-RMab® MUC1 Rabbit mAb at 1/500 dilution
Lane 1: HCT116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Negative control: HCT116 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 24 kDa

FC

Flow cytometric analysis of HCT116 (Human colorectal carcinoma epithelial cell, left) / T47D (Human ductal breast epithelial tumor epithelial cell, right) cells labelling S-RMab® MUC1 antibody at 1/250 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: HCT116

IP

MUC1 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating MUC1 in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using MUC1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: T-47D whole cell lysate 20 µg (Input)
Lane 2: MUC1 Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 17 kDa
Observed MW: 24 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in T-47D cells. Anti- MUC1 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

Negative control：ICC shows negative staining in HCT-116 cells.Anti- MUC1 antibody was used at 1/125 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

Immunofluorescence

IF shows positive staining in paraffin-embedded human colon cancer. Anti-MUC1 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human colon. Anti- MUC1 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human kidney. Anti-MUC1 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human breast cancer. Anti- MUC1 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.