Flow cytometric analysis of BALB/c mouse splenocytes labelling Mouse CD25 (IL-2Rα) antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Rat IgG1, λ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody.Then cells were stained with CD4 - Brilliant Violet 421™ Antibody separately.
Rat Anti-Mouse CD25 (IL-2Rα) Antibody (S-3558)
Rat Anti-Mouse CD25 (IL-2Rα) Antibody (S-3558)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra |
| Location | Membrane |
| Accession | P01590 |
| Clone Number | S-3558 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,λ |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
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