Flow cytometric analysis of BALB/c mouse splenocytes labelling Mouse CD2 antibody at 1/200 dilution (1 μg) / (Red) compared with a Rat IgG2b, κ Isotype Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Rat Anti-Mouse CD2 Antibody (S-R721)
Rat Anti-Mouse CD2 Antibody (S-R721)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD2 |
| Synonyms | T-cell surface antigen CD2; LFA-2; LFA-3 receptor; Lymphocyte antigen 37 (Ly-37); T-cell surface antigen T11/Leu-5; Ly-37 |
| Location | Cell membrane |
| Accession | P08920 |
| Clone Number | S-R721 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD2, also known as the T-cell rosette receptor or LFA-2, is a type I membrane protein predominantly expressed on thymocytes and mature T cells. It functions as an adhesion molecule that binds to CD58 (LFA-3) on antigen-presenting cells (APCs), facilitating T-cell receptor (TCR) binding and signal transduction, which is crucial for T-cell activation and immune responses.
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