WB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 70 kDa
PKC delta Recombinant Rabbit mAb (S-2611-39)
PKC delta Recombinant Rabbit mAb (S-2611-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PKC delta |
| Synonyms | Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Pkcd; Prkcd |
| Immunogen | Recombinant Protein |
| Location | Cell membrane, Cytoplasm, Nucleus, Mitochondrion |
| Accession | P28867 |
| Clone Number | S-2611-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | 293T, HeLa, K562, A431, NIH/3T3, mouse brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| IHC-P | 1:200-1:1000 | Hu, Ms |
| ICC | 1:100 | Hu |
Background
Protein kinase C delta (PKCδ) is a serine/threonine kinase of the novel PKC subfamily that, once activated by diacylglycerol or oxidative stress, translocates to distinct subcellular compartments where it phosphorylates substrates such as caspase-3, p53, and STAT1 to regulate apoptosis, DNA-damage responses, immune signaling, and tumor suppression, and its catalytic activity is fine-tuned by tyrosine phosphorylation (e.g., Y311) and by the formation of complexes with receptors, adaptors, and lipids, making PKCδ a central but context-dependent switch between survival and cell death.
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Western Blot
WB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 68 kDa
WB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 68 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PKC delta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-PKC delta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PKC delta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-PKC delta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PKC delta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PKC delta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in 293T cells. Anti- PKC delta antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
