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Phospho-ULK1 (Ser757) Recombinant Rabbit mAb (S-3495)

Phospho-ULK1 (Ser757) Recombinant Rabbit mAb (S-3495)

Catalog Number: S0B6445 Application: WB Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Phospho-ULK1 (Ser757)
Synonyms Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; hATG1); Unc-51-like kinase 1; KIAA0722; ULK1
Location Cytoplasm
Accession O75385
Clone Number S-3495
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Hu
Positive Sample A172
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu

Background

Phospho-ULK1 (Ser757) is the phosphorylated form of the serine/threonine kinase ULK1 at serine 757, a modification catalyzed by mTORC1 under nutrient-rich conditions that disrupts ULK1’s interaction with AMPK and keeps autophagy suppressed; upon starvation or rapamycin-triggered mTORC1 inhibition, Ser757 dephosphorylation enables AMPK binding and subsequent phosphorylation of activation-loop sites, licensing ULK1 kinase activity and initiating autophagosome formation.

Picture

Western Blot

WB result of Phospho-ULK1 (Ser757) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ULK1 (Ser757) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A172 whole cell lysate 20 µg
Lane 2: A172 treated with 250nM Torin 1 for 5 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 150 kDa