WB result of Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HepG2 whole cell lysate 20 µg
Lane 2: HepG2 treated with 50 ng/ml Human BMP2 for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb (S-3412)
Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb (S-3412)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) |
| Synonyms | Mothers against decapentaplegic homolog; MAD homolog; Mothers against DPP homolog; SMAD family member |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | Q99717、Q15797、O15198 |
| Clone Number | S-3412 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-SMAD1 (Ser463/465)/SMAD5 (Ser463/465)/SMAD9 (Ser465/467) proteins are the BMP-activated, C-terminally phosphorylated forms of the R-SMADs 1, 5 and 9 that, upon phosphorylation by activated type-I BMP receptors, form heteromeric complexes with co-SMAD4, translocate into the nucleus and directly bind to BMP-responsive promoter elements to orchestrate transcriptional programs governing cell fate, proliferation, differentiation and apoptosis across developmental and homeostatic contexts.
Picture
Picture
Western Blot
WB result of Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated MEF whole cell lysate 20 µg
Lane 2: MEF treated with 50 ng/ml Human BMP2 for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
