WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 75 kDa
Phospho-RIP (Ser166) Recombinant Rabbit mAb (S-2702-58)
Phospho-RIP (Ser166) Recombinant Rabbit mAb (S-2702-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-RIP (Ser166) |
| Synonyms | Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); Rinp; Rip; Ripk1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q60855 |
| Clone Number | S-2702-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-RIP (Ser166) is the phosphorylated form of receptor-interacting protein kinase 1 (RIPK1) at serine 166, a modification that acts as a biomarker for the kinase’s activation and marks the initiation of RIPK3/MLKL-dependent necroptotic signaling; this phosphorylation event converts RIPK1 from a pro-survival scaffold into a death-inducing kinase, enabling assembly of the necrosome complex (RIPK1–RIPK3–MLKL) that drives inflammatory, caspase-independent cell death, and its detection is therefore widely used to monitor necroptotic activity in cancer, neurodegeneration and inflammatory disease research.
Picture
Picture
Western Blot
WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 75 kDa
