WB result of Phospho-MLKL (Ser345) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-MLKL (Ser345) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
Phospho-MLKL (Ser345) Recombinant Rabbit mAb (S-2439-64)
Phospho-MLKL (Ser345) Recombinant Rabbit mAb (S-2439-64)
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$131.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-MLKL (Ser345) |
| Synonyms | Mixed lineage kinase domain-like protein; Mlkl |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | Q9D2Y4、Q8NB16 |
| Clone Number | S-2439-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-MLKL (Ser345) is the activated, phosphorylated form of the pseudokinase mixed-lineage kinase domain-like protein (MLKL) at serine-345, a critical post-translational modification catalyzed by the necroptotic kinase RIPK3 that triggers MLKL’s oligomerization, translocation to plasma membrane phosphoinositides, and subsequent membrane disruption, thereby executing the terminal, pro-inflammatory cell death pathway known as necroptosis; detection of phospho-MLKL(Ser345) by phospho-specific antibodies thus serves as a specific biomarker for ongoing necroptotic signaling in experimental models and human pathologies including ischemia–reperfusion injury, inflammatory diseases, and certain cancers.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb
Lane 1: MLKL (Ser345) phospho peptide
Lane 2: MLKL (Ser345) unmodified peptide
Primary antibody: Phospho-NAK/TBK1 (Ser172) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
