WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg (λ phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Phospho-Glycogen Synthase (Ser641) Rabbit Polyclonal Antibody
Phospho-Glycogen Synthase (Ser641) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Glycogen Synthase (Ser641) |
| Synonyms | Glycogen [starch] synthase, muscle; Glycogen synthase 1; GYS; GYS1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P13807 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, NIH/3T3, PC-12 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
Background
Phospho-Glycogen Synthase (Ser641) is the inactive, phosphorylated form of glycogen synthase in which a phosphate group is covalently attached to serine-641; this single modification, catalyzed chiefly by glycogen synthase kinase-3 (GSK-3) downstream of insulin signaling, sterically hinders the enzyme’s active site and prevents the elongation of glycogen chains, thereby providing a rapid, reversible switch that links hormonal cues to systemic glucose storage.
Picture
Picture
Western Blot
WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg (λ phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
WB result of Phospho-Glycogen Synthase (Ser641) Rabbit pAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Glycogen Synthase (Ser641) Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg (λ phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Dot Blot
Dot blot result of Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb
Lane 1: Glycogen Synthase (Ser641) phospho peptide
Lane 2: Glycogen Synthase (Ser641) unmodified peptide
Primary antibody: Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
