WB result of Phospho-EphA2 (Tyr588) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-EphA2 (Tyr588) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HepG2 whole cell lysate 20 µg
Lane 2: HepG2 treated with 10 μM MG132 for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 125 kDa
Phospho-EphA2 (Tyr588) Recombinant Rabbit mAb (S-3418)
Phospho-EphA2 (Tyr588) Recombinant Rabbit mAb (S-3418)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-EphA2 (Tyr588) |
| Synonyms | Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; ECK; EPHA2 |
| Location | Cell membrane |
| Accession | P29317 |
| Clone Number | S-3418 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-EphA2 (Tyr588) is the EphA2 receptor tyrosine kinase autophosphorylated on Tyr588, a modification that stabilizes the active kinase conformation, amplifies downstream Ras/MAPK, PI3K/Akt, RhoA and Src signaling cascades governing cell–cell repulsion, migration, invasion, anchorage-independent growth and angiogenesis, and thereby serves as a biomarker and potential therapeutic target in aggressive cancers.
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Western Blot
