WB result of Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 strave overnight, then treated with 100 ng/ml hM-CSF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb (S-3346)
Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb (S-3346)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD115 |
| Synonyms | Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; FMS; CSF1R |
| Location | Cell membrane |
| Accession | P07333 |
| Clone Number | S-3346 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
Background
CD115, also known as Colony-Stimulating Factor 1 Receptor (CSF1R) or M-CSF receptor (M-CSFR), is a 972-amino-acid cell-surface glycoprotein encoded by the CSF1R gene (c-FMS proto-oncogene) that belongs to the type III receptor tyrosine kinase family; it serves as a receptor for the cytokines CSF-1 and IL-34, and is highly expressed on monocyte-lineage cells and osteoclasts, mediating their survival, proliferation, differentiation, and motility through ligand-induced oligomerization and transphosphorylation, with mutations linked to myeloid malignancies and increased expression observed in Alzheimer’s disease microglia and breast carcinogenesis.
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Western Blot
