WB result of Phospho-Bad (Ser112) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bad (Ser112) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 strave overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 20 kDa
This blot was developed with high sensitivity substrate
Phospho-Bad (Ser112) Recombinant Rabbit mAb (S-3596)
Phospho-Bad (Ser112) Recombinant Rabbit mAb (S-3596)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Bad (Ser112) |
| Synonyms | Bcl2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-2-like protein 8 (Bcl2-L-8); Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BBC6; BCL2L8; BAD |
| Location | Cytoplasm, Mitochondrion |
| Accession | Q92934 |
| Clone Number | S-3596 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt, Mk |
Background
Phospho-Bad (Ser112) is the phosphorylated form of the pro-apoptotic Bcl-2 family member Bad at serine 112, a modification catalyzed by survival kinases such as PKB/Akt, p90RSK or Pim-2 that creates a 14-3-3-binding motif, sequestering Bad in the cytosol and thereby preventing it from neutralizing anti-apoptotic Bcl-2/Bcl-xL at the mitochondrial outer membrane; this single phosphorylation event thus functions as a molecular switch converting Bad from an apoptosis inducer to a silent scaffold, integrating growth-factor, stress and cytokine signals to set the apoptotic threshold in neurons, lymphocytes and many cancer cells.
Picture
Picture
Western Blot
