Flow cytometric analysis of Mouse NK1.1 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with APC Rat Anti-Mouse CD49b antibody and either PE-Cy7 Mouse IgG2a, κ Isotype Control (Left panel) or SDT PE-Cy7 Mouse Anti-Mouse NK1.1 Antibody (Right panel) at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Mouse Anti-Mouse NK1.1 Antibody (S-R480)
PE-Cy7 Mouse Anti-Mouse NK1.1 Antibody (S-R480)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | NK1.1 |
| Synonyms | Killer cell lectin-like receptor subfamily B member 1B allele A; CD161 antigen-like family member B; Lymphocyte antigen 55b (Ly-55b); NKR-P1 34; Natural killer cell surface protein NKR-P1B allele SJL/BALB (NKR-P1B); CD161b; Ly55b; Nkrp1b; Klrb1b |
| Accession | P27812 |
| Clone Number | S-R480 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
NK1.1, also known as KLRB1C and NKRP1C, is a type II integral membrane glycoprotein with a C-type lectin domain and belongs to the NKR-P1 family of cell surface receptors. It is expressed as a disulfide-linked homodimer on all NK and NKT cells in some strains of mice, particularly serving as a good marker for these cell types in the C57BL/6 genetic background. NK1.1 associates with the ITAM-bearing γ-chain adaptor molecule and does not engage ligands with an MHC class-I-like fold, but interacts with C-type lectin-like CLEC2 glycoproteins. Although crosslinking of the NK1.1 receptor using a specific mAb can induce NK cell-mediated cytotoxicity and effector cytokine secretion, the in vivo functions of NK1.1 and the identity of its ligand(s) are still not fully understood.
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