Flow cytometric analysis of Human CD69 expression on PHA stimulated human PBMC (human peripheral blood mononuclear cell). Human PBMC were either not stimulated (Left panel) or stimulated 2 days with 10 μg/ml PHA (Right panel) and then were stained with SDT Pacific Blue Mouse Anti-Human CD69 Antibody at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse Anti-Human CD69 Antibody (S-2878)
Pacific Blue Mouse Anti-Human CD69 Antibody (S-2878)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD69 |
| Synonyms | Early activation antigen CD69; Activation inducer molecule (AIM); BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; CLEC2C |
| Location | Cell membrane |
| Accession | Q07108 |
| Clone Number | S-2878 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | PHA stimulated human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD69 is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily. It is a classical early marker of lymphocyte activation, appearing rapidly on the cell surface after stimulation. Structurally, CD69 is a disulfide-linked homodimer protein with two differentially glycosylated subunits (28–32 kDa), each consisting of an extracellular C-type lectin domain connected to a single-spanning transmembrane region followed by a short cytoplasmic tail. It is broadly expressed on the surfaces of most hematopoietic lineages, including activated T and B lymphocytes, natural killer (NK) cells, murine macrophages, neutrophils, and eosinophils, and is constitutively expressed on human monocytes, platelets, epidermal Langerhans cells, and bone-marrow myeloid precursors. CD69 plays various regulatory roles in immune responses, such as promoting tissue residency, regulating Th17/Treg cell differentiation, and contributing to the exhaustion of resident memory T cells, especially in the tumor microenvironment. Its ligands include galectin-1, calprotectin (S100A8/S100A9 complex), myosin light chains 9, 12a, and 12b (Myl9/12), and oxidized low-density lipoprotein (ox-LDL).
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