Flow cytometric analysis of Human CD44 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Pacific Blue Mouse IgG1, κ Isotype Control (Black line histogram) or SDT Pacific Blue Mouse Anti-Human CD44 Antibody (Red line histogram) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse Anti-Human CD44 Antibody (S-630-174)
Pacific Blue Mouse Anti-Human CD44 Antibody (S-630-174)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD44 |
| Synonyms | CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; LHR; MDU2; MDU3; MIC4 |
| Location | Cell membrane |
| Accession | P16070 |
| Clone Number | S-630-174 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD44 is a multifunctional transmembrane glycoprotein that functions as a major adhesion molecule for hyaluronan and other extracellular matrix components, playing critical roles in cell migration, proliferation, differentiation, and survival; it is expressed in numerous isoforms generated through alternative splicing, with its standard form (CD44s) and variant isoforms (CD44v) exhibiting distinct tissue distributions and functional properties, and its dysregulated expression has been implicated in various pathological processes including inflammation, autoimmune diseases, and cancer progression where it contributes to tumor cell invasion, metastasis, and resistance to apoptosis.
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