WB result of p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb
Primary antibody: p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 39 kDa
p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb (S-3860)
p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb (S-3860)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | p38 delta/MAPK13 + p38 alpha/MAPK14 |
| Synonyms | Mitogen-activated protein kinase; MAP kinase; MAPK |
| Location | Cytoplasm, Nucleus |
| Accession | Q16539、O15264 |
| Clone Number | S-3860 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, A431, NIH/3T3 |
| Predicted Reactivity | BV, Dg, Hm, AfGrMk |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
MAPK13 and MAPK14, commonly known as p38δ and p38α respectively, are critical members of the p38 mitogen-activated protein kinase (MAPK) signaling pathway. Functioning as essential intracellular signaling molecules, they are primarily activated in response to diverse stress stimuli, such as inflammatory cytokines, environmental stress, and oxidative stress. Their activation depends on the dual phosphorylation of specific threonine and tyrosine residues, which in turn regulates the activity of numerous downstream transcription factors and proteins. MAPK14 (p38α) is the most extensively studied and ubiquitously expressed isoform, playing a central role in processes like inflammatory responses, apoptosis, cell differentiation, and cell cycle regulation, making it a significant drug target for inflammatory and autoimmune diseases. In contrast, MAPK13 (p38δ) exhibits a more tissue-specific expression pattern, predominantly found in glandular tissues (e.g., salivary glands, pancreas), skin, and lungs, where it fulfills distinct roles in tissue-specific inflammation, skin pathophysiology, and the regulation of insulin secretion. Although they share upstream activators and some downstream substrates, MAPK13 and MAPK14 exhibit both overlapping and complementary physiological functions, collectively forming a core network for the precise cellular regulation of stress responses.
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Western Blot
WB result of p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb
Primary antibody: p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 39 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human esophagus. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
