WB result of NRF1 Recombinant Rabbit mAb
Primary antibody: NRF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 68 kDa
NRF1 Recombinant Rabbit mAb (S-2409-103)
NRF1 Recombinant Rabbit mAb (S-2409-103)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NRF1 |
| Synonyms | Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein (Alpha-pal); NRF1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q16656 |
| Clone Number | S-2409-103 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, MCF7, PC-12 |
| Predicted Reactivity | Zf |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
NRF1 (Nuclear Respiratory Factor 1) is a ubiquitously expressed transcription factor that functions as a master regulator of nuclear-encoded mitochondrial genes by binding to GC-rich promoter elements, thereby orchestrating mitochondrial biogenesis, oxidative phosphorylation, and cellular redox homeostasis; it also controls the expression of key components of the proteasome and lysosome systems, maintains mitochondrial DNA transcription and replication, and its activity is modulated by post-translational modifications and subcellular localization, making it essential for energy metabolism, cell survival, and adaptation to metabolic stress.
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Picture
Western Blot
WB result of NRF1 Recombinant Rabbit mAb
Primary antibody: NRF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 68 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-NRF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
