WB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25 kDa
MMP7 Recombinant Rabbit mAb (S-1628-25)
MMP7 Recombinant Rabbit mAb (S-1628-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MMP7 |
| Synonyms | Matrilysin; Matrin; Matrix metalloproteinase-7 (MMP-7); Pump-1 protease; Uterine metalloproteinase; MPSL1; PUMP1 |
| Immunogen | Recombinant Protein |
| Location | Secreted, Extracellular space |
| Accession | P09237 |
| Clone Number | S-1628-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HT-29, BxPC-3, A549 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
Matrix metalloproteinase-7 (MMP-7) is a small proteolytic enzyme that is secreted as a zinc- and calcium-dependent endopeptidase. It plays a crucial role in the degradation of various extracellular matrix substrates and non-ECM proteins, such as E-cadherin and Fas ligand, which are involved in cell adhesion and apoptosis. MMP-7 is implicated in several physiological processes, including wound healing, bone growth, and remodeling. In cancer, MMP-7 is often overexpressed and contributes to tumor progression by promoting cell proliferation, invasion, metastasis, and angiogenesis. Its expression levels correlate with the clinical characteristics of cancer patients, making it a potential biomarker and therapeutic target for various types of cancer.
Picture
Picture
Western Blot
WB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 23, 25 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human liver. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
