WB result of MFG1-E8 Recombinant Rabbit mAb
Primary antibody: MFG1-E8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: PANC-1 whole cell lysate 20 µg
Lane 6: human milk lysate 20 µg
Low expression control: Daudi whole cell lysate; HL-60 whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35-48 kDa
MFG1-E8 Recombinant Rabbit mAb (S-2530-74)
MFG1-E8 Recombinant Rabbit mAb (S-2530-74)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MFG1-E8 |
| Synonyms | Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8 (MFG-E8); SED1; MFGE8 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | Q08431 |
| Clone Number | S-2530-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | A549, PANC-1, human milk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Milk fat globule-EGF factor 8 (MFG-E8), also called lactadherin, is a secreted 66–75 kDa glycoprotein composed of an N-terminal EGF-like domain containing an RGD integrin-binding motif and two C-terminal discoidin/F5-8 domains that bind phosphatidylserine, enabling it to act as a bridging molecule between apoptotic cells and phagocytes expressing αvβ3/αvβ5 integrins, thereby orchestrating efferocytosis, dampening inflammation, and exerting pleiotropic effects on vascular remodeling, skeletal-muscle lipid metabolism, myogenic differentiation, osteoclastogenesis, and tumor progression while also giving rise to the amyloidogenic peptide medin found in aged arterial walls.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in PANC-1 cells. Anti- MFG1-E8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
