WB result of LILRB4 Recombinant Rabbit mAb
Primary antibody: LILRB4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 62 kDa
LILRB4 Recombinant Rabbit mAb (S-2504-22)
LILRB4 Recombinant Rabbit mAb (S-2504-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LILRB4 |
| Synonyms | Leukocyte immunoglobulin-like receptor subfamily B member 4; B4; CD85 antigen-like family member K; Immunoglobulin-like transcript 3 (ILT-3); Leukocyte immunoglobulin-like receptor 5 (LIR-5); Monocyte inhibitory receptor HM18; CD85k; ILT3; LIR5 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q8NHJ6 |
| Clone Number | S-2504-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | THP-1, HL-60 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
| IHC-P | 1:500 | Hu |
Background
LILRB4 (Leukocyte Immunoglobulin-Like Receptor B4), also known as ILT3, is a type I transmembrane protein with two extracellular Ig-like domains and three intracellular ITIM motifs that recruit SHP-1/2 phosphatases to dampen myeloid-cell activation; expressed chiefly on monocytes, macrophages and dendritic cells, it functions as a key immune checkpoint that maintains self-tolerance by inhibiting NF-κB/MAPK signaling, suppressing T-cell proliferation and promoting Treg/Ts differentiation, but is exploited by tumors such as AML through ligands like ApoE to enable immune evasion, leukemia invasion and poor prognosis, making it an emerging therapeutic target in cancer, autoimmunity and transplantation.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human anaplastic lymphoma. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
