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Human Anti-Mouse F4/80 Antibody (S-3458)

Human Anti-Mouse F4/80 Antibody (S-3458)

Catalog Number: S0B8072 Application: FCM Reactivity: Mouse Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Human
Antigen F4/80
Synonyms Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1
Location Cell membrane
Accession Q61549
Clone Number S-3458
Antibody Type Recombinant mAb
Isotype IgG1,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse peritoneal exudates cells
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1:2000 Ms

Background

F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.

Picture

FC

Flow cytometric analysis of BALB/c mouse peritoneal exudates cells labelling Mouse F4/80 antibody at 1/2000 dilution (0.1 μg) / (Right panel) compared with a Human IgG1, λ Isotype Control / (Left panel). Goat Anti-Human IgG Alexa Fluor® 488 was used as the secondary antibody.Then cells were stained with CD11b - Brilliant Violet 421™ Antibody separately.