WB result of Glycogen Synthase Recombinant Rabbit mAb
Primary antibody: Glycogen Synthase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A204 whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
Glycogen Synthase Recombinant Rabbit mAb (S-2698-24)
Glycogen Synthase Recombinant Rabbit mAb (S-2698-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glycogen Synthase |
| Synonyms | Glycogen [starch] synthase, muscle; Glycogen synthase 1; GYS; GYS1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P13807 |
| Clone Number | S-2698-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, HepG2, Jurkat, A204, HEK-293 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
Glycogen Synthase (GS) is the key rate-limiting enzyme in the glycogen biosynthesis pathway, primarily responsible for catalyzing the elongation of the glycogen chain. It uses uridine diphosphate glucose (UDP-Glucose) as the activated glucose donor, adding glucose molecules via α-1,4-glycosidic bonds to a pre-existing glycogen primer chain, thereby linearly extending the glucose polymer. The activity of this enzyme is precisely regulated by both allosteric control and covalent modification (reversible phosphorylation): the insulin signaling pathway activates its activity through dephosphorylation, promoting glycogen storage; whereas stress hormones like glucagon and adrenaline cause its inactivation through phosphorylation, thereby inhibiting glycogen synthesis and mobilizing energy release. Thus, Glycogen Synthase is a central molecule in maintaining the body's glucose homeostasis, and its aberrant activity is closely associated with metabolic diseases such as Type II diabetes and insulin resistance.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
