WB result of Gelsolin Rabbit pAb
Primary antibody: Gelsolin Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A204 whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 90 kDa
Gelsolin Rabbit Polyclonal Antibody
Gelsolin Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Gelsolin |
| Synonyms | AGEL; Actin-depolymerizing factor (ADF); Brevin; GSN |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Secreted |
| Accession | P06396 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HeLa, A204, PANC-1, C2C12, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Mk |
Background
Gelsolin is a highly conserved, multifunctional actin-binding protein that severs, caps and nucleates actin filaments in response to Ca²⁺ and phosphoinositide signals, thereby orchestrating rapid cytoskeletal remodeling essential for cell motility, endocytosis, apoptosis and immune responses; it exists as both an 80–82 kDa cytoplasmic isoform and a slightly larger secreted plasma variant generated by alternative transcription initiation, each contributing to tissue repair and host defense by clearing actin filaments released from damaged cells, and its dysregulation—through mutation, proteolytic cleavage or altered expression—is implicated in cancer metastasis, neurodegeneration, amyloidosis and other human diseases.
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Western Blot
WB result of Gelsolin Rabbit pAb
Primary antibody: Gelsolin Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 90 kDa
WB result of Gelsolin Rabbit pAb
Primary antibody: Gelsolin Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 90 kDa
